Covalent and non-covalent binding in the ion/ion charge inversion of peptide cations with benzene-disulfonic acid anions

Immobilization of Subtilisin Carlsberg on Modified Silica Gel by Cross-linking and Covalent Binding Methods

Proteases are important enzymes that their role in various industries is undeniable. However, keeping enzymes stable during its activity in harsh conditions is so important. In this study, protease enzyme was immobilized on the porous silica particles and its stability in different temperatures and pHs was evaluated. First silica particles were aminated by 3-aminopropyltriethoxysilane then the ...

Kinetics of residual chloride transport in human red blood cells after maximum covalent 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid binding

Irreversible inhibition, 99.8% of control values for chloride transport in human red blood cells, was obtained by well-established methods of maximum covalent binding of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). The kinetics of the residual chloride transport (0.2%, 106 pmol.cm-2 x s-1) at 38 degrees C, pH 7.2) was studied by means of 36Cl- efflux. The outside apparent affinity, ...

Electrostatic and non-covalent interactions in dicationic imidazolium-sulfonium salts with mixed anions.

A series of thioether-functionalised imidazolium salts have been prepared and characterized. Subsequent reaction of the thioether-functionalised imidazolium salts with iodomethane affords imidazolium-sulfonium salts composed of doubly charged cations and two different anions. Imidazolium-sulfonium salts containing a single anion type are obtained either by a solvent extraction method or by anio...

Gold nanoparticle self-assembly promoted by a non-covalent, charge-complemented coiled-coil peptide

Charge Up in Wired Covalent Organic Frameworks

Crystalline porous covalent organic frameworks (COFs) with suitable redox-active sites were found to be useful as electrodes for capacitive energy storage. However, the bottleneck issues of COFs, i.e., their poor electrical conductivity and instability, greatly limit their practical implementation. Now, a strategy has been developed to overcome these obstacles and enable the use of COFs for hig...